Format: 96-well plate

Form: 500 Tests

Detection Method: Colorimetric

Sensitivity: 1000 cells (adherent); 2500 cells (nonadherent)

Assay Range: 1000 - 50,000 Cells

Assay Time: 0.5 - 4 h

Sample Type: Cultured cells

Kit Contents: WST-1, 1-Methoxy PMS, and a user protocol.

Comments: Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.

Ref.: Ishiyama, M., et al. 1995. In Vitro Toxicology 8, 187. Liu, S.O., et al 1995. Nat. Med. 1, 267. Ishiyama, M., et al. 1993. Chem. Pharm. Bull. 41, 1118.