| Format: 24-well plate | | Form: 12 Tests | | Detection Method: Fluorescence | | Assay Time: 24-48 h | | Sample Type: Culture cells | | Kit Contents: 24-Well Plate containing 12 cell culture inserts coated with Basement Membrane Extract, Cell Detachment Solution, Cell Staining Solution, Cell Staining Diluent, and a user protocol. | | Comments: A convenient and useful assay for the quantitative measurement of cell invasion. Contains 12 cell culture inserts with an 8 µm polycarbonate membrane. This membrane is coated with a thin layer of a biological matrix. This layer prevents noninvasive cells from going through the membrane. Cells are quantified utilizing a highly sensitive fluorescent dye (Excitation: ~485 nm; Emission: ~520 nm). | | Ref.: Montell, D.J. 2003. Nat. Rev. Mol. Cell Biol. 4, 13. Egblad, M., et al. 2002. Nat. Rev. Cancer 2, 161. Hood, J.D., et al. 2002. Nat. Rev. Cancer 2, 91. Chatterjee, N., et al. 2001. Environ. Pathol. Toxicol. Oncol. 20, 211. Koul, D., et al. 2001. Oncogene 20, 6669. Kleinman, H.K., et al. In Molecular and Cellular Aspects of Basement Membranes, Rohrbach, D.H. and Timpl, R., eds. Academic Press, 1993, pp. 309. Repesh, L.A. 1989. Invasion Metastasis 9, 192. Albini, A., et al. 1987. Cancer Res. 47, 3229. Terranova, V.P., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 465. Liotta, L.A., 1984. Am. J. Pathol. 117, 339. | |