Calbiochem: QIA18: p21WAF1 ELISA Kit

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��	p21^WAF1 ELISA Kit

Cat. No. QIA18��

All Categories � Calbiochem � Kits and Tools � Cell Cycle and Cell Proliferation Assays

CIP1 ELISA Kit
p21 ELISA Kit
SD11 ELISA Kit
WAF ELISA Kit

Format: 96-well plate
Form: 96 Tests
Detection Method: Colorimetric
Species Reactivity: human
Sensitivity: 0.1 U/ml
Assay Range: 0.1 - 20 U/ml
Unit Definition: One unit (U) is defined as the amount p21^WAF1 measured from 2.7 x 10⁵ cells.
Assay Time: 4 h
Sample Type: Cell extracts or tissue culture media
Kit Contents: Coated 96-Well Removable Strip Plate, 2 vials of p21^WAF1 Standard, Antigen Extraction Agent, Sample Diluent, Biotinylated Monoclonal Anti-p21^WAF1 Detector Antibody, Streptavidin Peroxidase Conjugate, Conjugate Diluent, Substrate, Plate Wash Concentrate, Stop Solution, Plate Sealers, and a user protocol.
Comments: The p21^WAF1 ELISA is a non-isotopic immunoassay for the in vitro quantitation of human p21 ^WAF1 protein in cell extracts or tissue culture media.
Ref.: Agarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493 Chen, Y.Q., et al. 1995. Int. J. Oncology 7, 889. Deng, C., et al. 1995. Cell 82, 675. El-Deiry, W.S., et al. 1995. Cancer Res. 55, 2910. Waldman, T., et al. 1995. Cancer Res. 55, 5187. Elbendary, A., et al. 1994. Cell Growth Diff. 5, 1301. El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Li, R., et al. 1994. Nature 371, 534. Michieli, P., et al. 1994. Cancer Res. 54, 3391 Noda, A., et al. 1994. Exp. Cell Res. 211, 90. El-Deiry, W.S., et al. 1993. Cell 75, 817. Gu, Y., et al. 1993. Nature 366, 707. Harper, J.W., et al. 1993. Cell 75, 805. Xiong, Y., et al. 1993. Genes Dev. 7, 1572. Xiong, Y., et al. 1993. Nature 366, 701. Xiong, Y., et al. 1992. Cell 71, 505.
R: 23/24/25-35-36/37/38-40-43; S: 26-36/37/39-45
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Graph. Induction of HL-60 differentiation by PMA leads to p53-independent up-regulation of p21^WAF1. p21^WAF1 is present at low levels in control (data not shown) and in retinoic acid-stimulated HL-60 cells. Differentiation of HL-60 cells with PMA along the macrophage pathway results in a 200-fold increase in p21^WAF1 within 1 hour of PMA treatment followed by a slow decline over 9 days. p21^WAF1 levels were measured with Cat. No. QIA18. Elevation of Nuclear Matrix Protein, detected with Cat. No. QIA20, over time demonstrates active apoptosis.

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