| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Species Reactivity: human | | Sensitivity: 10 U/ml | | Assay Range: 15-1000 U/ml | | Unit Definition: One unit (U) is defined as the amount of NMP 41/7 released by 850 MCF7 cells maintained at a density of 1 - 5 x 105 cells/ml in serum-free medium for 7 days. | | Assay Time: 4 h | | Sample Type: Tissue culture supernatant | | Kit Contents: Coated 96-Well Removable Strip Plate, Lyophilized NMP Standard, NMP Detector Antibody, Anti-FITC-POD, Substrate, ELISA Conjugant Diluent, 20X Plate Wash Concentrate, Stop Solution, Plate Sealer, and a user protocol. | | Comments: Designed for the quantitative detection of NMP41/7. The specific detection of soluble human NMPs afforded by the Cell Death Detection (Nuclear Matrix Protein) ELISA allows quantitation of cell death. | | Ref.: Miller, T.E., et al. 1994. In Current Communications in Cellular and Molecular Biology: Apoptosis II. 8, 357. Arguello, F., et al. 1993. Blood 82, 999a. Miller, T.E., et al. 1993. Biotechniques 15, 1042. Partin, A.W., et al. 1993. Cancer Res. 53, 744. Compton, D.A., et al. 1992. J. Cell Biol. 116, 1395. Miller, T.E., et al. 1992. Cancer Res. 52, 422. Yang, C.H., et al. 1992. J. Cell Biol. 116, 1303. Nakayasu, H. and R. Berezney. 1989. J. Cell Biol. 108, 1. Fey, E.G. and S. Penman. 1988. Proc. Natl. Acad. Sci. USA 85, 121. Zeitlin, S., et al. 1987. Mol. Cell Biol. 7, 111. Kumara-Siri, M.H. 1986. Biol. Chem. 261, 2844. Berrios, M., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 4142. Pardoll, D.M., et al. 1980. Cell 19, 527. Berezney, R. and D.S. Coffey. 1974. Biochem. Biophys. Res. Commun. 60, 1410. | | Use of this product is covered by U.S. Patents 5,783,403, 5,882,876, 5,989,826, 6,162,607, 6,410,247, and 6,740,494. Foreign patents also cover the use of this product in numerous jurisdictions. |
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