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 | -20°C |  | Dry ice |  | Hazardous |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| |  | Cat. No. QIA23 |
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| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Species Reactivity: human | | Sensitivity: < 1 ng/ml | | Assay Time: 3.5 h | | Sample Type: Cell lysates, tissue culture supernatant, sera, or plasma | | Kit Contents: 96 Well Coated Plate, Antigen Extraction Reagent, Lyophilized Bcl-2 Standard, Sample Diluent, Anti-FITC Conjugated Detector Antibody, Concentrated Anti-FITC Peroxidase Conjugate, Conjugate Diluent, Substrate, Wash Buffer, Stop Solution, Adhesive Plate Sealers, and a user protocol. | | Comments: A quantitative colorimetric assay for the measurement of Bcl-2. Able to detect significant decreases in Bcl-2 protein levels before significant levels of apoptosis are detected. Kit has high sensitivity, a broad dynamic range, and contains all required buffers. | | One unit of Bcl-2 equals the Bcl-2 activity in 5.6 X 104 HL-60 cells | | Ref.: Korsmeyer, S.J., 1995. Trends Genet. 11, 101. Hockenbery D.M., 1995. BioEssays 17, 631. Steinman, H.M., 1995. J. Biol. Chem. 270, 3487. Hawkins, C.J., and Vaux, D., 1994. Immunol. Rev. 142, 127. Hengartner, M.O., et al. 1994. Nature 369, 318. Ellis, R.E., et al. 1994. Soc. London Ser. 34, 243. Zoltan, N., et al. 1994. Cell 79, 189. Tsujimoto, Y. 1989. Oncogene 4, 1331. | | R: 23/24/25-35-36/37/38-40-43; S: 26-36/37/39-45 |
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* Additional Product Information
Dilution Recovery With Different AEA. Dilution recovery from a bcl-2 positive cell line using different antigen extraction agents (different detergents).
Dilution Recovery With Different Cell Lines. Dilution recovery using different cell lines.
Neutralization Assay. Neutralization of bcl-2 positive samples using a bcl-2 monoclonal antibody (Cat. No. OP60).
Precision. The pooled coefficient of variation (according to the formula of Henry, et al., 1974) is plotted against bcl-2 concentrations. The pooled data were collected from samples run (in replicates of four) on two separate occasions using two different lots of coated plates on each occasion (total of four assays). The triangles represent samples run on one occasion.
Precision. The pooled coefficient of variation (according to the formula of Henry, et al., 1975) is plotted against bcl-2 concentrations. The pooled data were collected from samples run (in replicates of four) on two separate occasions using two different lots of coated plates on each occasion (total of four assays). The triangles represent samples run on one occasion.
Recovery in Human Sera and Plasma. Human sera and plasma samples were spiked with bcl-2 protein to obtain a final concentration of 42 units/ml and analyzed as outlined above. The control represents bcl-2 spiked into sample diluent.
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