| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Species Reactivity: human | | Specificity: Human cathepsin D protein | | Sensitivity: 4 ng/ml | | Assay Range: 4-100 ng/ml | | Assay Time: 5 h | | Sample Type: Tissue cytosol extracts or cell culture extracts | | Kit Contents: Pre-Coated Plate, 2 vials of Lyophilized Standard, Detector Antibody, 400X Conjugate, Conjugate Diluent, Antigen Extraction Agent, Colorimetric Detection Reagent, Sample Diluent, 20X Plate Wash Concentrate, Stop Solution, Plate Sealer, and a user protocol. | | Comments: A rapid, sensitive assay for the measurement of human cathepsin D. | | Ref.: Kute, T.E., et al. 1992. Can. Res. 52, 5198. Maudelonde, T., et al. 1992. Eur. J. Cancer 28A, 1686. Rochefort, H. 1992. Eur. J. Cancer 28A, 1780. Merkel, D.E., et al. 1991. Breast Cancer Research and Treatment 19, 200. Garcia, M., et al. 1990. Oncogene 5, 1809. Henry, J.A., et al. 1990. Cancer (Phila.) 65, 265. Rochefort, H. In: Seminars in Cancer Biology 1990. (M.M. Gottesman, Ed.) Vol. 1(2) 153. Tandon, A.K., et al. 1990. N. Eng. J. Med. 322, 297. Spyratos, F., et al. 1989. Lancet 334, 1115. Briozzo, P., et al. 1988. Can. Res. 48, 3688. Garcia, M., et al. 1986. Can. Res. 46, 3734. Vignon, F., Capony, F., et al. 1986. Endocrinol. 118, 1537. Bradford, M.M. 1976. Anal. Biochem. 72, 248. Smith, P.K., et al. 1985. Anal. Biochem. 150, 7. Westley, B. and Rochefort, H. 1980. Cell 20, 353. | |