TUNEL Assay

Format: Flow cytometry or fluorescence microscopy

Form: 50 Tests

Detection Method: Fluorescence

Species Reactivity: a broad range of species

Assay Time: 2 h

Sample Type: Frozen or paraffin sections, fixed cell preparations or cell suspensions

Kit Contents: Proteinase K, Euilibration and Lbeling Buffers, TdT Enzyme (Cat. No. PF060), Control Slides, Mounting Media (Cat. No. HC08), and a user protocol.

Comments: Allows for detection of apoptosis at the individual cell level. Apoptosis morphology is easily detected. Detection is by flow cytometry or fluorescence microscopy. The kit mounting media permits the observance of the total cell population.

Cat. No. QIA39 is a fluorescein-conjugated version of our widely referenced TdT Colorimetric FragEL™ DNA Fragmentation Detection Kit (Cat. No. QIA33) for non-isotopic labeling. Terminal deoxynucleotidyl transferase (TdT) binds to exposed 3′-OH ends of DNA fragments generated in response to apoptotic signals and catalyzes the addition of fluorescein-labeled and unlabeled deoxynucleotides. When excited, fluorescein generates an intense signal which can be detected by fluorescence microscopy or flow cytometry. The mounting media sustains the fluorescent signal from samples labeled on slides and, therefore, aids in the morphological evaluation and characterization of normal and apoptotic cells. Non-apoptotic cells can be visualized by a DAPI filter.

Ref.: Darzynkiewicz, Z., et al. 1997. Cytometry 27, 1. Frey, T. 1997. Cytometry 28, 253. Shapiro, H.M. 1995. Practical Flow Cytometry, Third Edition. Wiley-Liss Inc., New York, New York. Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795. Gavrieli, Y., et al. 1992. J. Cell Biol. 119, 493. Fawthrop, D. J., et al. 1991. Arch. Toxicol. 65, 437. Martin, S.J., et al. 1990. J. Immunology 145, 1859. Wyllie, A. H. 1980. Nature 284, 555. Kerr, J. F. R., et al. 1972. Br. J. Cancer 26, 239.

R: 23/25-34-36/37/38-42/43-45-61; S: 26-27-36/37/39-45-53