| Format: 96-well strip plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Species Reactivity: human, mouse, rat | | Specificity: Human PCNA protein | | Sensitivity: 0.5 U/ml of PCNA | | Assay Range: 1.875 - 30 U/ml | | Unit Definition: One unit (U) is defined as the amount of PCNA measured from ~ 2.6 x 103 SW620 cells. | | Assay Time: 3.5 h | | Sample Type: Cell lysates, tissue culture media , serum, or plasma | | Kit Contents: 96-Well Removable Strip Plate, 2 vials of PCNA Standard, Detector Antibody, 400X Conjugate, Conjugate Diluent, Substrate, Sample Diluent, 20X Plate Wash Concentrate, Antigen Extraction Agent, Stop Solution, Plate Sealers, and a user protocol. | | Comments: Fast, sensitive assay for the in vitro quantitation of PCNA protein. The kit has broad reactivity, and is suitable to detect PCNA in autoimmune sera samples. The assay was validated using the BrdU Cell Proliferation Assay (Cat. No. QIA58). | | Proliferating cell nuclear antigen (PCNA) has no known enzymatic activity; however, it can interact with a variety of proteins and regulate their activities. These varied interactions with DNA metabolic proteins imply that PCNA is a central factor for the coordination of DNA replication, DNA repair, epigenetic inheritance, and cell-cycle control. | | Ref.: Kelman, Z. and J. Hurwitz, 1998. Trends Biochem. Sci. 23, 236. Montecucco, A., et al. 1998. The EMBO Journal 17, 3786. Oku, T., et al. 1998. Genes Cells 3, 357. Waga, S. and B. Stillman, 1998. Annu. Rev. Biochem. 67, 721. Jonsson ZO and H. U, 1997. Bioessays 19, 967. Prosperi, E., 1997. Prog. Cell Cycle Res. 3, 19. Gulbis, J., et al. 1996. Cell, 87, 297. Brott, D.A., et al. 1993. J. Cell. Biochem. 52, 362. | | R: 23/24/25-35-36/37/38-40-43; S: 26-36/37/39-45 |
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