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 | -20°C |  | Dry ice |  | Hazardous |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Species Reactivity: human | | Sensitivity: 5 pg/ml | | Assay Range: 0-1000 pg/ml | | Assay Time: 4 h | | Sample Type: Serum, cell lysates, tissue culture supernatants, and plasma samples | | Kit Contents: Antibody Coated 96-Well Plate, TGF-a Standards, Reporter Antibody, Peroxidase Conjugate, Peroxidase Substrate, Stop Solution, Rinse Buffer, Antigen Extraction Agent, Assay Buffer, Plate Sealers, and a user protocol. | | Ref.: Ellis, D.L., et al. 1990. J. Invest. Dermatol. 95, 27. Katoh, M., et al. 1990. Biochem. Biophys. Res. Commun. 167, 1065. Derynck, R., 1988. Cell 54, 593. Coffey, R.J., et al. 1987. Nature 328, 817. Yeh, Y.C., et al. 1987. Cancer Res. 47, 896. Derynck, R., 1986. J. Cell Biochem. 32, 203. Samsoondar, J., et al. 1986. J. Biol. Chem. 261, 14408. Lee, D.C., et al. 1985. Molec. Cell. Biol. 5, 3644. Todaro, G.J., et al. 1985. In Genes and Protein in Oncogenesis, Vogel, H., Weinstein, I.B. (Eds.) New York, Raven Press pp. 165. Anzano, M.A., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 6264. Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4684. Reynolds, F.H., Jr., et al. 1983. Nature 292, 259. |
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* Additional Product Information
Graph. Phorbol ester and concanavalin A induced up-regulation of TGF-a in MDA MB 231 cells. TGF-a is synthesized and transported to the cell surface as a membrane-anchored precursor (proTGF-a) that is converted to the soluble form by proteolytic cleavage. ProTNF-a cleavage is activated in response to tumor-promoting phorbol ester. Concanavalin A is a mitogen known to up-regulate cytokine production in numerous cell lines.
Standard Curve. The mean signal of each standard run in replicates of three in eight assays using two different lots of plates and two different lots of detector antibody.
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