Human Fibroblast Growth Factor, Basic ELISA Kit | Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Species Reactivity: human | | Specificity: Recognizes both natural and recombinant human bFGF | | Sensitivity: 3 pg/ml | | Assay Range: 10-320 pg/ml | | Assay Time: 5 h | | Sample Type: Cell culture supernatants, serum, plasma. | | Kit Contents: Coated 96-Well Plate, bFGF Conjugate, bFGF Standard, Assay Diluent, Calibrator Diluent, Wash Buffer Concentrate, Color Reagent A, Color Reagent B, Stop Solution, Plate Sealers, and a user protocol. | | Comments: Applicable for the in vitro quantitation of bFGF in cell culture supernatants, serum, and plasma. | | Ref.: Baird, A. and M. Klagsbrun. 1991. Cancer Cells A Monthly Review 3:1991. Bugler, B. et al. 1991. Mol. Cell. Biol. 11, 573. Klagsbrun, M. 1991. The New Biologist 3, 745. Mignatti, P. et al. 1991. Proc. Natl. Acad. Sci. USA 88, 11007. Quarto, N. et al. .1991. J. Cell. Physiol. 147, 311. Baird, A. and P. Bohlen. 1990. "Fibroblast Growth Factors" in Peptide Growth Factors and Their Receptors I, Sporn, M.B. and A.B. Roberts, eds. Springer Verlag, New York, p. 369. Basilico, C. et al. 1989. Ann. N. Y. Acad. Sci. 567, 95. Burgess, W.H. and T. Maciag. 1989. Ann. Rev. Biochem. 58, 575. Mignatti, P. et al. .1989. J. Cell Biol. 108, 671. Prats, H. et al. 1989. Proc. Natl. Acad. Sci. USA 86, 1836. Rogelj, S. et al. 1989. J. Cell. Biochem. 39, 13. Thomas, K. 1987. Methods Enzymol. 147, 120. | |