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 | Multiple Storage Requirements |  | Multiple conditions |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| | Caspase-3 Activity Assay Kit, Fluorometric |
|  | Cat. No. QIA70 |
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| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Fluorometric | | Species Reactivity: a broad range of species | | Assay Time: 2.5-3 h | | Sample Type: Cell lysates | | Kit Contents: 96-Well Plate, Caspase-3 Extraction Buffer, Assay Buffer, Recombinant Caspase-3, Caspase-3 Substrate Conjugate, Caspase-3 Inhibitor, Control Cell Pellets, and a user protocol. | | Comments: Suitable for the in vitro detection of caspase-3 activity in cell lysates. The assay is simple, rapid, and includes all controls. Suitable for use in frozen samples. Assay is validated against the Nucleosome ELISA Kit (Cat. No. QIA25) and the Cell Death Detection NMP ELISA Kit (Cat. No. QIA20). A high-throughput drug-screening version is also available (Cat. No. HTS02). | | Ref.: X. Sun, et al. 1999. J. Biol. Chem. 274, 5053. N.A. Thornberry and Y. Lazebnik, 1998. Science 281, 1312. S. J. Martin and D. 1995 R. Green, Cell 82, 349. | | R: 36/37/38; S: 26-36-23 |
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Graph. Caspase-3 activity of rat splenocytes stimulated with T cell mitogens concanavalin A (Con A) (Cat. No. 234567), phytohemagglutinin (PHA), and Phorbol Myristate Acetate (Cat. No. 524400) + Calcium Ionophore (Cat. No. 401952) (PMA/Ion). Fresh isolated rat splenocytes were treated with T cell mitogens and caspase-3 activity was determined at the indicated time points. For the first 24 hours, there was little change in caspase-3 activity. At the 48-hour time point, caspase-3 activity of Con A-stimulated splenocytes started to dramatically increase while the caspase activity of the splenocytes stimulated by the other two mitogens decreased.
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| - So-Young Park and Adriana Ferreira. (2005) The generation of a 17 kDa neurotoxic fragment: an alternative mechanism by which Tau mediates β-amyloid-induced neurodegeneration. Journal of Neuroscience 25, 5365-5375.
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