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Kit Resource
  Caspase-8 Activity Assay Kit, Fluorometric
Cat. No. QIA71  
All Categories » Calbiochem » Kits and Tools » Apoptosis Activity and Detection Kits » Caspase

CAP4 Assay Kit
CASP8 Assay Kit
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MACH Assay Kit
Mch5 Assay Kit

Format: 96-well plate
Form: 96 Tests
Detection Method: Fluorometric
Species Reactivity: a broad range of species
Assay Time: 2-3 h
Sample Type: Cell lysate
Kit Contents: 96-Well Plate, Caspase-8 Extraction Buffer, Assay Buffer, Recombinant Caspase-8, Caspase-8 Substrate Conjugate, Caspase-8 Inhibitor, Control Cell Pellets, and a user protocol.
Comments: Suitable for the in vitro detection of caspase-8 activity in cell lysates. The assay is simple, rapid, and includes all controls. Suitable for use in frozen samples. Assay is validated against the Cell Death Detection NMP ELISA Kit (Cat. No. QIA20).
Ref.: X.Sun, M., et al. 1999. J. Biol. Chem. 274, 5053. Thornberry, N.A. and Lazebnik, Y. 1998. Science 281, 1312. Martin, S.J. and Green, D.R. 1995. Cell 82, 349.
R: 22-36/37/38-41; S: 23-26-36

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Graph. Specificity of the signal. The signals of the drug-treated samples in the presence of a specific inhibitor of caspase-8 are very similar and very low. This is consistent with the concept that most of the signal is due to caspase-8 activity.

Related Literature:

Caspases and other Apoptosis Related Tools Brochure

Antibodies for Cancer Research Product Guide

Tools and Tips for Analyzing Apoptosis

Material Safety Data Sheets:
QIA71: Caspase-8 Activity Assay Kit, Fluorometric - English
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All Categories » Calbiochem » Kits and Tools » Apoptosis Activity and Detection Kits » Caspase