| Format: Fluorescence microscopy |
| Form: 100 Tests |
| Detection Method: Fluorescence |
| Species Reactivity: a broad range of species |
| Assay Time: 0.5 h |
| Sample Type: Cell suspensions or adherent cells |
| Kit Contents: Staining Buffer, Cyto-Dye, Propidium Iodide, and a user protocol. |
| Comments: This kit uses a cell-permeable green fluorescent Cyto-dye (Ex. max.: 488 nm; Em. max.: 518 nm) to stain live cells, and propidium iodide (Ex. max.: 488 nm; Em. max.: 615 nm) to stain dead cells. Stained live and dead cells can be visualized by fluorescence microscopy using a band-pass filter which detects FITC and rhodamine. Viable cells will stain only with the Cyto-dye, fluorescing green, whereas the dead cells will stain with both Cyto-dye (green) and propidium iodide (red), resulting in a yellow fluorescence. Note: 1 T = 1 test. |
| Optimal staining conditions may vary among different cell types and should be determined empirically by the investigator. |
| Ref.: Luther, E., and Kamenstsky, L.A. 1996. Cytometry 23, 272. Frey. T., et al. 1995. Cytometry 21, 265. |
| R: 36/37/38-46; S: 26-36/37/39-45 |
