Format: Flow cytometry or fluorescence microscopy

Form: 100 Tests

Detection Method: Fluorescence

Species Reactivity: a broad range of species

Assay Time: 1.5 h

Sample Type: Cultured cells

Kit Contents: Labeled Caspase-3 Inhibitor (Red-DEVD-FMK), Wash Buffer, Caspase-3 Inhibitor (Z-VAD-FMK), and a user protocol.

Comments: A convenient and sensitive method for the detection of activated caspase-3 in living cells. The fluorescent marker is DEVD-FMK conjugated to sulforhodamine. This cell-permeable, non-toxic inhibitor binds irreversibly to activated caspase-3 in apoptotic cells. The fluorescence intensity can be measured by fluorescence microscopy, fluorescence microplate reader, or flow cytometry.

R: 36/37/38; S: 23-26-36