Calbiochem: QIA97: Cu/Zn Superoxide Dismutase ELISA Kit

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��	Cu/Zn Superoxide Dismutase ELISA Kit

Cat. No. QIA97��

All Categories � Calbiochem � Kits and Tools � Oxidative Stress Research Tools and Assays

Format: 96-well plate
Form: 96 Tests
Detection Method: Colorimetric
Species Reactivity: human
Sensitivity: 40 pg/ml
Assay Time: 1.5 h
Sample Type: Cell culture supernatant, serum, plasma, urine, amniotic fluid, and other biological fluids
Kit Contents: Pre-Coated 96-Well Plate, Anti-Cu/Zn SOD/HRP-Conjugate, Cu/Zn SOD Standard, Wash Buffer Concentrate, Assay Buffer Concentrate, Phosphate Buffered Saline, Substrate Solution, Blue and Green Dyes, Stop Solution, Plate Covers, and a user protocol.
Comments: A colorimetric sandwich ELISA method for detecting Cu/Zn superoxide dismutase (SOD) in different types of biological samples.
Ref.: Herouart, D., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3108. Niwa, Y., et al. 1993. Am. J. Pathol. 143, 312. Suzuki, H., et al. 1993. J. Clin. Invest. 91, 2727. Holzgreve, W., et al. 1991. Diagnose Labor. 41, 162. Porstmann, T., et al. 1991. Prenat. Diagn. 11, 195. Shull, S., et al. 1991. J. Biol. Chem. 266, 24398. Porstmann, T., et al. 1990. Hum. Genet. 85, 362. Porstmann, T., et al. 1988. Clin. Chim. Acta 171, 1. Jolly, S.R., et al. 1984. Circ. Res. 54, 277. Goebel, K.M. and Storck, U. 1983. Am. J. Med. 74, 124. Barra, D., et al. 1980. FEBS Lett. 120, 53. Sinet, P.M., et al. 1976. Exp. Cell Res. 97, 47. Harta, J.W. and Deutsch, H.F. 1972. Biol. Chem. 247, 7043.
R: 36/37/38; S: 26-36-45

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Inter-Assay Reproducibility. Data represents the mean Cu/Zn SOD concentration and the coefficient of variation calculated on 18 determinations of each sample. The overall inter-assay coefficient of variation was determined to be 5.8%.

Intra-Assay Reproducibility. Data represents the mean Cu/Zn SOD concentration and the coefficient of variation for each sample. The overall intra-assay coefficient of variation was determined to be 5.1%.