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| |  | Cat. No. SC1000 |
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Anti-Early Embryo Specific Expression NK Family Gene
Anti-ENK
Anti-Nanog Homeobox | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids within residues 1-50 of mouse Nanog | | Form: Liquid | | Formulation: In Tris-citrate/phosphate buffer, pH 7.0-8.0. | | Preservative: ≤0.1% sodium azide | | Positive Control: Mouse myeloid embryonic stem cells | | Comments: Recognizes the ~40 kDa Nanog protein in mouse myeloid and embryonic stem cells. | | Ref.: Chambers, I., et al. 2003. Cell 113, 643. Mitsui, K., et al. 2003. Cell 113, 631. Pan, G.J. and Pei, D.Q. 2003. Cell Res. 13, 499. |
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Panel A: Detection of mouse Nanog by immunoblotting. Samples: Whole cell lysate from mouse myleoid cells (15 µg, lane 1) and mouse embryonic stem cells (5 µg, lane 2). Primary antibody: Anti-Nanog Rabbit pAb (Cat. No. SC1000) (0.1 µg/ml). Detection: chemiluminescence. Panel B: Detection of mouse Nanog by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from mouse embryonic stem cells. Antibody for IP: Anti-Nanog Rabbit pAb (Cat. No. SC1000) (1 µg/mg total protein). Immunoblotting conditions: same as panel A.
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