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| | PhosphoDetect™ Anti-FAK (pTyr407) Rabbit pAb |
|  | Cat. No. ST1010 |
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Anti-Focal Adhesion Kinase, Phospho-Specific (Tyr407) | Note: 1 T = 1 test. | | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide surrounding the Tyr407 phosphorylation site of human FAK | | Form: Liquid | | Formulation: In Dulbecco's PBS, 1 mg/ml BSA, pH 7.4. | | Preservative: None | | Positive Control: Trichostatin-treated NIH 3T3 cells | | Comments: Recognizes the ~125 kDa FAK protein phosphorylated at Tyr407. | | Ref.: Lim, Y., et al. 2002. J. Biol. Chem. 277, 12735. Datta, A., et al. 2001. Mol. Cell. Biol. 21, 7295. Vial, D., et al. 2000. J. Biol. Chem. 275, 28269. Tamura, M., et al. 1999. J. Biol. Chem. 274, 20693. |
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EMD Chemicals Inc. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| | chicken, frog, human, mouse | IB, IC
See Key |
Detection of chicken FAK, phosphorylated at Tyr407, by immunoblotting. Sample: Whole cell lysate from chick embryo fibroblasts expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr407) Rabbit pAb (Cat. No. ST1010) pre-incubated without peptide (lane 1), or with phosphopeptide immunogen (lane 2), non-phosphopeptide corresponding to the immunogen (lane 3), or a generic phosphotyrosine-containing peptide (lane 4). (0.3 µg/ml). Detection: chemiluminescence.
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