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 | ≤ -70°C |  | Dry ice | | Note: Store and Ship conditions may differ. | | See Key |
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| | PhosphoDetect™ Anti-GSK-3a/b (pTyr279/216) Rabbit pAb |
|  | Cat. No. ST1013 |
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Anti-Glycogen Synthase Kinase-3a/b, Phospho-Specific (Tyr279/216) | Note: 1T = 1 test. Sufficient for 10 Western miniblots. | | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr279/216 phosphorylation sites of human GSK-3a/b | | Form: Liquid | | Formulation: In Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3. | | Preservative: ≤0.1% sodium azide | | Positive Control: Serum starved insulin-treated 3T3-L1 cells | | Negative Control: Normal rabbit serum | | Comments: Recognizes the ~51 kDa GSK-3a protein phosphorylated at Tyr279 and the ~47 kDa protein GSK-3b phosphorylated at Tyr216. | | Ref.: Papasozomenos, S.C., et al. 2002. PNAS 99, 1140. Kirschenbaum, F., et al. 2001. J. Biol. Chem. 276, 7366. Bhat, R.V., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11074. |
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EMD Chemicals Inc. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| | human, mouse, rat | DB, ELISA, IB, IC
See Key |
Detection of human GSK-3b, phosphorylated at Tyr216 by immunoblotting. Samples: Whole cell lysate from control cells (lane 1) or control lysate spiked with human recombinant GSK-3b (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Glycogen Synthase Kinase-3a/b (pTyr279/216) Rabbit pAb (Cat. No. ST1013) (1:1000) pre-incubated without peptide (lanes 1, 5), or with phosphopeptide immunogen (lane 2), a generic phosphoserine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence.
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