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  PhosphoDetect™ Anti-MEK1 (pSer298) Rabbit pAb
Cat. No. ST1015  
All Categories » Calbiochem » Antibodies » Primary Antibodies » Kinases » MAPK

Note: 1T = 1 test. Sufficient for 10 Western miniblots.
Host: Rabbit
Isotype: IgG
Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser298 phosphorylation site of human MEK1
Form: Liquid
Formulation: In Dulbecco's PBS, 1 mg/ml BSA, pH 7.3.
Preservative: ≤0.1% sodium azide
Positive Control: NIH3T3 or A431 cells treated with PDGF
Comments: Recognizes the ~43 kDa MEK1 protein phosphorylated at Ser298 in PDGF-treated NIH3T3 and A431 cells.
Ref.: Frost, J.A., et al. 1997. EMBO J. 16, 6426.
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10 TYN/A

CloneSpecies ReactivityApplication
 human, mouse, ratIB 
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Detection of mouse MEK1, phosphorylated at Ser298, by immunoblotting. Samples: Whole cell lysate from NIH3T3 cells left untreated (lane 1) or treated with PDGF (lanes 2-7). Primary antibody: PhosphoDetect™ Anti-MEK1 (pSer298) Rabbit pAb (Cat. No. ST1015) (1:1000) pre-incubated without peptide (lanes 1, 2, 6, 7), or with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.

Related Literature:

Protein Kinase and Related Tools Brochure
PhosphoDetect™ Phospho-specific Antibodies Brochure

Material Safety Data Sheets:
ST1015: PhosphoDetect™ Anti-MEK1 (pSer298) Rabbit pAb - English
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All Categories » Calbiochem » Antibodies » Primary Antibodies » Kinases » MAPK
 
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are brands of EMD Chemicals Inc., an Affiliate of Merck KGaA, Darmstadt, Germany.