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| | PhosphoDetect™ Anti-Phospholipase Cg1 (pTyr783) Rabbit pAb | |  | Cat. No. ST1020 | |
Anti-PLCg1, Phospho-Specific (Tyr783) | Note: 1T = 1 test. Sufficient for 10 Western minoblots. | | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr783 phosphorylation site of human PLCg1 | | Form: Liquid | | Formulation: In Dulbecco's PBS, 1 mg/ml BSA, 50% glycerol, pH 7.2-7.4. | | Preservative: ≤0.1% sodium azide | | Positive Control: NIH3T3 cells treated with PDGF | | Comments: Recognizes the ~135 kDa PLCg1 protein phosphorylated at Tyr783 in PDGF-treated NIH3T3 cells. | | Ref.: Irvin, B.J., et al. 2000. Mol. Cell. Biol. 20, 9149. Law, C-L., et al. 1996. Mol. Cell. Biol. 16, 1305. | |
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| | bovine, human, mouse, rat, Xenopus | IB, IC See Key |
 Detection of mouse Cg1, phosphorylated at Tyr783, by immunoblotting. Sample: Whole cell lysate from untreated (left panel) and PDGF treated (10 ng/ml, 10 min) NIH3T3 cells (right panel). Primary antibody: PhosphoDetect™ Anti-Phospholipase Cg1 (pTyr783) Rabbit pAb (Cat. No. ST1020) (1:1000).
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