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 | 2 to 8°C |  | Blue ice |  | Highly Toxic | | Note: Store and Ship conditions may differ. | | See Key |
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| |  | Cat. No. ST1042 |
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Anti-Protein Kinase D2 | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids near the C-terminus of human PKD2 | | Form: Liquid | | Formulation: In Tris-citrate/phosphate buffer, pH 7.0-8.0. | | Preservative: ≤0.1% sodium azide | | Positive Control: HEK 293 cells | | Comments: Recognizes the ~105 kDa PKD2 protein in HEK293 cells. | | Ref.: Rey, O., et al. 2003. Biochem. Biophys. Res. Commun. 302, 817. Sturany, S., et al. 2002. J. Biol. Chem. 277, 29431. Sturany, S., et al. 2001. J. Biol. Chem. 276, 3310. |
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Panel A: Detection of human PKD2 by immunoblotting. Samples: Whole cell lysate (30 µg) from untransfected HEK 293 cells (Ctrl, left lane), HEK 293 cells transfected with PKD3-GFP fusion protein (~136 kDa, middle lane), or HEK 293 cells transfected with PKD1-GFP fusion protein (~136 kDa, right lane). Primary antibody: Anti-PKD2 Rabbit pAb (Cat. No. ST1042) (1:1000) Detection: chemiluminescence. Panel B: Detection of human PKD2 by immunoprecipitation, followed by immunoblotting. Sample: Whole cell lysate (200 µg protein) from HEK 293 cells. Antibody for IP: Anti-PKD2 Rabbit pAb (Cat. No. ST1042) (1:100). Immunoblotting conditions: same as above.
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