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| | Anti-20S Proteasome b4-Subunit (72-85) Rabbit pAb |
|  | Cat. No. ST1056 |
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Anti-C7
Anti-Proteasome Subunit b type 2 | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids 72-85 of human proteasome subunit b4, conjugated to KLH | | Form: Liquid | | Formulation: In PBS. | | Preservative: ≤0.1% sodium azide | | Positive Control: HeLa cell lysate | | Comments: Recognizes the ~23 kDa 20S proteasome b4-subunit in HeLa cells. | | Ref.: Kopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 2939. |
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Detection of human 20S proteasome b4 subunit by immunoblotting. Samples: Purified human erythrocyte-derived 20S proteasome (Lanes a,b) and HeLa S3 S100 cytosolic preparation (Lanes c,d). Primary antibody: Anti-20S Proteasome b4-subunit (72-85) Rabbit pAb (Cat. No. ST1056) (1:1000) pre-incubated without blocking peptide (Lanes a,c) or with blocking peptide (Lanes b,d).
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