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| |  | Cat. No. ST1063 |
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Anti-Regulatory Associated Protein of mTOR | Host: Rabbit | | Isotype: IgG | | Immunogen: a recombinant protein consisting of a C-terminal fragment of human Raptor | | Form: Liquid | | Formulation: In PBS. | | Preservative: ≤ 0.1% sodium azide | | Positive Control: HEK293 cells | | Comments: Recognizes the ~150 kDa Raptor protein in HEK293 cells. | | Ref.: Choi, K.M., et al. 2003. J. Biol. Chem. 278, 19667. Schalm, S.S., et al. 2003. Curr. Biol. 13, 797. Hara, K., et al. 2002. Cell 110, 177. | | S: A09 |
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Detection of human Raptor by immunoblotting and immunoprecipitation. Samples: Whole cell lysate (80 µg) from HEK293 cells (lane 1) and Raptor immunoprecipitated from 1 mg total protein from HEK293 whole cell lysates (lane 2) (10 µg Anti-Raptor Rabbit pAb, Cat. No. ST1063). Primary antibody for immunoblotting: Anti-Raptor Rabbit pAb (Cat. No. ST1036) (1 µg/ml). Detection: chemiluminescence.
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