 |
 | -20°C |  | Blue ice | | Note: Store and Ship conditions may differ. | | See Key |
|
|
|
|
| | PhosphoDetect™ Anti-Raf (pSer259) Rabbit pAb |
|  | Cat. No. ST1076 |
|
| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser259 phosphorylation site of human c-Raf | | Form: Liquid | | Formulation: In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | | Preservative: None | | Positive Control: HeLa cells treated with TPA | | Comments: Recognizes the ~74 kDa c-Raf protein phosphorylated at Ser259. May detect an additional protein at ~68 kDa. | | Ref.: Weber, S.M., et al. 2002. J. Immunol. 168, 5303. Cieslik, K., et al. 2001. J. Biol. Chem. 276, 1211. Zimmermann, S. and Moelling, K. 1999. Science 286, 1741. Sprenkle, A.B., et al. 1997. FEBS Lett. 403, 254. |
|
 | Related information for this product is available: | |
| |
EMD Chemicals Inc. GBP list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| | human, mouse, rat | IB, IP, PS
See Key |
Detection of human Raf, phosphorylated at Ser259, by immunoblotting. Samples: Whole cell lysate from untreated (-) or 200 nM TPA (+) (Cat. No. 524400) treated HeLa cells. Primary antibodies: PhosphoDetect™ Anti-Raf (pSer259) Rabbit pAb (Cat. No. ST1076) (Top) and Anti-c-Raf (Bottom). Detection: chemiluminescence.
|
|
|
