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| | PhosphoDetect™ Anti-IKKa/b (pSer176/180) Rabbit pAb |
|  | Cat. No. ST1078 |
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Anti-IKB Kinase a/b, Phospho-Specific (Ser176/180) | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids surrounding the Ser176/180 phosphorylation sites of human IKKa | | Form: Liquid | | Formulation: In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | | Preservative: None | | Positive Control: HeLa cells treated with TNFa (Cat. No. 654205) or calyculin A (Cat. No. 208851) | | Comments: Recognizes the ~85 kDa IKKa protein phosphorylated at Ser176/180 and the ~87 kDa IKKb protein phosphorylated at Ser177/181 in TNF-a- or calyculin A-treated HeLa cells. | | Ref.: Dai, S., et al. 2004. J. Biol. Chem. 279, 37219. Delhase, M., et al. 1999. Science 284, 309. |
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Detection of human IKKa/b, phosphorylated at Ser176/180, by immunoblotting. Samples: Whole cell lysate from HeLa cells treated with TNF-a (Cat. No. 654205) (20 ng/ml for 5 min) or Calyculin A (Cat. No. 208851) (50 nM). Primary antibodies: PhosphoDetect™ Anti-IKKa/b (pSer176/180) Rabbit pAb (Cat. No. ST1078) (1:1000) (top), Anti-IKKa (middle), or Anti-IKKb (bottom). Detection: chemiluminescence.
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