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| | PhosphoDetect™ Anti-Tuberin/TSC2 (pThr1462) Rabbit pAb |
|  | Cat. No. ST1084 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids surrounding the Thr1462 phosphorylation site of human Tuberin/TSC2 | | Form: Liquid | | Formulation: In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | | Preservative: None | | Positive Control: NIH3T3 cells treated with PDGF | | Comments: Recognizes the ~200 kDa tuberin/TSC2 protein phosphorylated at Thr1462 in PDGF=treated NIH3T3 cells. | | Ref.: Tee, A.R., et al. 2003. J. Biol. Chem. 278, 37288. Liu, M.Y., et al. 2002. Cancer Res. 62, 6475. |
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Detection of human and mouse tuberin/TSC2, phosphorylated at Thr1462, by immunoblotting. Samples: Whole cell lysate from NIH 3T3 cells that were left untreated, or treated with PDGF (Cat. No. 521200) (50 ng/ml), PDGF and wortmannin (Cat. No. 681675) (200 nM), or PDGF and rapamycin (Cat. No. 553210) (5 nM), and MCF7 cells that were untreated or IGF-1 (Cat. No. 407240) for the indicated times. Primary antibodies: PhosphoDetect™ Anti-Tuberin/TSC2 (pThr1462) Rabbit pAb (Cat. No. ST1084) (1:1000) (upper) or Anti-Tuberin/TSC2 (lower). Detection: chemiluminescence.
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