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 | 2 to 8°C |  | Blue ice |  | Highly Toxic | | Note: Store and Ship conditions may differ. | | See Key |
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| | PhosphoDetect™ Anti-WASP (pSer483/484) Rabbit pAb |
|  | Cat. No. ST1087 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser483/484 phosphorylation sites of human WASP | | Form: Liquid | | Formulation: In Tris-citrate/phosphate buffer, pH 7.0-8.0. | | Preservative: ≤0.1% sodium azide | | Positive Control: Jurkat or U937 cells | | Comments: Recognizes the ~60 kDa WASP protein phosphorylated at Ser483/484 in U937 cells. May detect an additional protein at ~110 kDa. | | Ref.: Millard, T.H., et al. 2004. Biochem. J. 380, 1. Cory, G.O.C., et al. 2003. Mol. Cell 11, 1229. |
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Detection of human WASP, phosphorylated at Ser483/484 by immunoblotting. Samples: Whole cell lysate (50 µg) from U937 cells left untreated (lane 1) or treated with lambda phosphatase (lane 2). Primary antibody: PhosphoDetect™ Anti-WASP (pSer483/484) Rabbit pAb (Cat. No. ST1087) (1:1000). Detection: chemiluminescence.
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