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| |  | Cat. No. ST1100 |
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Anti-Exportin
Anti-Chromosome region maintenance 1 protein | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids in the C terminal region of human CRM1 | | Form: Liquid | | Formulation: In Tris-citrate/phosphate bufer, pH 7-8. | | Preservative: ≤ 0.1% sodium azide | | Positive Control: HeLa cells | | Comments: Recognizes the ~100 kDa human CRM1 protein in HeLa cells. | | Ref.: Kudo, N., et al. 1998. Exp. Cell Res. 242, 540. Fornerod, M., et al. 1997. Cell 90, 1051. Kudo, N., et al. 1997. J. Biol. Chem. 272, 29742. |
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Lane 1: Detection of human CRM1 by immunoblotting. Sample: Whole cell lysate (50 µg) from HeLa cells. Primary antibody: Anti-CRM1 Rabbit pAb (Cat. No. ST1100) (1 µg/ml). Detection: chemiluminescence. Lane 2: Detection of human CRM1 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from HeLa cells. Antibody for IP: Anti-CRM1 Rabbit pAb (ST1100) (5 µg/500 µg total protein in 500 µl). Immunoblotting conditions: same as Lane 1.
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