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| |  | Cat. No. ST1102 | |
Anti-Importin-b1 Anti-Kapb1 Anti-Karyopherin-b1 Anti-Nuclear factor p97 | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids near the C-terminus of human Kpnb1 | | Form: Liquid | | Formulation: In Tris-citrate/phosphate buffer, pH 7-8. | | Preservative: ≤0.1% sodium azide | | Positive Control: Jurkat cells | | Comments: Recognizes the ~97 kDa Kpnb1 protein in Jurkat cells. | | Ref.: Chook, Y.M. and Blobel. G. 2001. Curr. Opin. Struct. Biol. 11, 703; Miroianu, J., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 6532; Gorlich, D., et al. 1995. Nature 377, 246. | | S: A09 | |
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| | human, mouse, Xenopus | IB, IP See Key |
 Lane 1: Detection of human Kpnb1 by immunoblotting. Sample: Whole cell lysate (50 µg) from Jurkat cells. Primary antibody: Anti-Kpnb1 Rabbit pAb (Cat. No. ST1102) (0.3 µg/ml). Detection: chemiluminescence. Lane 2: Detection of human Kpnb1 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Antibody for IP: Anti-Kpnb1 Rabbit pAb (Cat. No. ST1102) (5 µg/500 µg total protein in 500 µl). Immunoblotting conditions: same as Lane 1.
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