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| |  | Cat. No. ST1112 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: recombinant, full length, human Rap1Gap fused to a His•Tag® sequence | | Form: Liquid | | Formulation: In 20 mM PBS. | | Preservative: None | | Positive Control: Bovine brain high speed supernatant or Ins 1 (832/13) cells expressing recombinant Rap1Gap protein | | Comments: Recognizes the ~80 kDa Rap1Gap protein in bovine brain (high speed centrifugation supernatant) and INS-1 (832/13) cells expressing recombinant Rap1Gap protein. | | Ref.: Wittchen E.S., et al. 2005. J. Biol. Chem. 280, 11675. Meng, J. and Casey, P.J. 2002. J. Biol. Chem. 277, 43417. |
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Detection of Rap1Gap by immunoblotting. Samples: Purified recombinant Rap1Gap (2 ng, lane 1; 5 ng, lane 2), whole cell lysate (25 µg) from Ins 1 (832/13) cells expressing Rap1Gap (lane 3), and bovine brain high speed supernatant (25 µg) (lane 4). Primary antibody: Anti-Rap1Gap Rabbit pAb (Cat. No. ST1112) (1:1000). Detection: chemiluminescence.
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