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| |  | Cat. No. ST1117 |
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Anti-NES | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids from an internal region of human NES | | Form: Liquid | | Formulation: In Tris-citrate/phosphate buffer, pH 7.0-8.0. | | Preservative: ≤0.1% sodium azide | | Positive Control: SHSY-5Y cells | | Comments: Recognizes the ~220 kDa Nestin protein in SHSY-5Y cells. | | Ref.: Shih, A.H. and Holland, E.C. 2006. Neoplasia 8, 1072. Singh, S.K., et al. 2004. Oncogene 23, 7267. |
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EMD Chemicals Inc. EUR list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
Lane 1: Detection of human nestin by immunoblotting. Sample: Whole cell lysate (20 µg) from SHSY-5Y cells. Primary antibody: Anti-Nestin Rabbit pAb (Cat. No ST1117) (0.5 µg/ml). Detection: chemiluminescence. Lanes 2 and 3: Detection of human nestin by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from (500 µg) from SHSY-5Y cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-Nestin Rabbit pAb (Cat. No. ST1117) (5 µg). Immunoblotting conditions: same as lane 1.
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