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| | Anti-ESPL1/Separase Rabbit pAb |
|  | Cat. No. ST1118 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids in the middle region of human separase. | | Form: Liquid | | Formulation: In Tris-citrate/phosphate buffer, pH 7.0-8.0. | | Preservative: ≤0.1% sodium azide | | Positive Control: HepG2 cells | | Comments: Recognizes the ~220 kDa ESPL1/separase protein in HepG2 cells. | | Ref.: Gorr, I.H., et al. 2006. Nat. Cell. Biol. 8, 1035. Holland, A.J. and Taylor, S.S. 2006. J. Cell Sci. 119, 3325. Kudo, N.R., et al. 2006. Cell 126, 135. |
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Lane 1: Detection of human separase by immunoblotting. Sample: Whole cell lysate (20 µg) from HepG2 cells. Primary antibody: Anti-Separase Rabbit pAb (Cat. No. ST1118) (1 µg/ml). Detection: chemiluminescence. Lane 2: Detection of human separase by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from HepG2 cells. Antibody for immunoprecipitation: Anti-Separase Rebbit pAb (Cat no. ST1118) (5 µg/500 µg total protein). Immunoblotting conditions: Sample: half of the immunoprecipitated separase. Primary antibody: Anti-Separase Rabbit pAb (Cat No. ST1118) (2 µg/ml). Detection: chemiluminescence.
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