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| | Anti-NOD2/CARD15 Rabbit pAb | |  | Cat. No. ST1119 | |
Anti-ACUG Anti-BLAU Anti-CARD15 | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids from human NOD2 | | Form: Liquid | | Formulation: In Tris-citrate/phosphate buffer, pH 7.0-8.0. | | Preservative: ≤0.1% sodium azide | | Comments: Recognizes the ~150 kDa NOD2 protein in Jurkat cells. | | Ref.: Fritz, J.H., et al. 2006. Nat. Immunol. 7, 1250. Radford-Smith, G. and Pandeya, N. 2006. World J. Gastroenterol. 12, 7097. | |
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 Lane 1: Detection of human NOD2 by immunoblotting. Sample: Whole cell lysate (20 µg) from Jurkat cells. Primary antibody: Anti-NOD2 Rabbit pAb (Cat. No. ST1119) (2 µg/ml). Detection: chemiluminescence. Lane 2. Detection of human NOD2 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from Jurkat cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-NOD2 Rabbit pAb (Cat. No. ST1119) (5 µg). Immunoblotting conditions: same as lane 1.
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