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| | Anti-FOXP3 Mouse mAb (3G3) | |  |
|  | Cat. No. ST1137 |
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| Host: Mouse | | Isotype: IgG1 | | Immunogen: full-length, recombinant, mouse FOXP3 containing a His•Tag® sequence | | Epitope: Within the N-terminus | | Form: Liquid | | Formulation: In PBS, 0.05% BSA. | | Preservative: ≤ 0.1% sodium azide | | Positive Control: Lymph node cells | | Comments: Recognizes the ~47 kDa FOXP3 protein in lymph node cells. | | Ref.: Gavin, M.A., et al. 2006.Proc Natl Acad Sci USA 17, 6659. | | S: A09 |
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Detection of human FOXP3 by immunoblotting. Samples: whole cell lysate from HEK293 cells transfected with full-length FOXP3 (lane 1) or mock-transfected (lane 2). Primary antibody: Anti-FOXP3 Mouse mAb (3G3) (Cat. No. ST1137) (2 µg/ml). Detection: chemiluminescence.
Detection of human FOXP3 by flow cytometry. Samples: mouse lymph node cells (2x106 cells). Primary antibodies: Anti-FOXP3 Mouse mAb (3G3) (Cat. No. ST1137) (100 ng in 50 µl total volume) (upper panel) and Anti-CD4 control antibody (lower panel). Detection: fluorescence.
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